RNA extraction from cells cultured on ACCs

We have tried to perform RNA extraction from MSC growing and differentiating cells using several protocols. The protocol described here is the adaptation from the manufacturer’s instructions with which we were able to successfully extract RNA from MSC cells growing and differentiating on a carbon cloth matrix.

Commercial kit used: Purelink RNA Mini Kit (Life Technologies, 12183018A). Manufacturer’s full protocol is available on their webpage.

Continue reading “RNA extraction from cells cultured on ACCs”